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. 2013 Mar 14;8(3):e59099. doi: 10.1371/journal.pone.0059099

Figure 1. A map of the plasmid constructions.

Figure 1

pAgpdi: the gpd promoter with the gpd first intron derived from A. bisporus. egfp: enhanced green fluorescent protein. hph: hygromycin B phosphotransferase gene derived from E. coli. P2A: 2A peptides derived from porcine teschovirus-1. poly A: nopaline synthase polyA signal. LB: left border. RB: right border. The star indicates the BamHI restriction enzyme site and the arrowhead indicates the BglII restriction enzyme site used for southern blot analysis.