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. 2013 Mar 14;8(3):e59099. doi: 10.1371/journal.pone.0059099

Figure 3. Expression of EGFP in F. velutipes transformants.

Figure 3

(A) Western blot analysis of EGFP protein expression with the various gene positions in the F. velutipes transformants AiH-P2AG and AiG-P2AH. (B) Western blot analysis of EGFP protein expression with the various copy numbers in the indicated F. velutipes transformants. Lane P: positive control, a commercial EGFP containing tags at both the N and C terminus (293 amino acids, 32.7 kDa), the EGFP expressed in transformants is 266 amino acids, 27 kDa; lane W: wild type; lane V-1, V-2: F. velutipes AiH transformant vector control; lane 1–1, 1–2: F. velutipes AiH-P2AG transformants; lane 2–1, 2–4: F. velutipes AiH-P2AGx2 transformant; lane 3–1, 3–2: F. velutipes AiH-P2AGx3 transformants; lane 4–1, 4–2: F. velutipes AiH-P2AGx4 transformants; and lane M: marker.