| Api-f |
5′-CCAAGCTTTTAAGAGGTCCGCAAGTAG-3′
|
Agaricus bisporus gpd promoter with |
| Api-r |
5′-CGCTGCAGCTACAAGTCGACATCAGTG-3′
|
the first intron cloning |
| P2A-f |
5′-CGGGATCCGCCTTCCAAGGCCCCGGCGCCACCAACTTCTCTCTCCTC-3′
|
2A peptides from porcine |
| P2A-r |
5′-GGGCCCGGGGTTCTCCTCGACGTCGCCGGCCTGCTTGAGGAGAGAGAA-3′
|
teschovirus-1 cloning |
| eGFP-f |
5′-GCTGCAGGTGAGCAAGGGCGAGGAGC-3′
|
egfp cloning |
| eGFP-r |
5′-GGGTCACCTTAAGATCTCTTGTACAGCTCGTCCAT-3′
|
|
| eGFP-362f |
5′-TGAACCGCATCGAGCTGAAGGG-3′
|
Real-time PCR analysis |
| eGFP-468r |
5′-ACCTTGATGCCGTTCTTCTGCTTG-3′
|
|
| eGFP-QF |
5′-GATGTTGTGGCGGATCTT-3′
|
Quantitative RT-PCR |
| eGFP-QR |
5′-TTGTACTCCAGCTTGTGC-3′
|
|
| Hyg-f |
5′-CGCTGCAGAAAAAGCCTGAACTCACC-3′
|
hph cloning |
| Hyg-r |
5′-CGGGATCCCTATTTCTTTGCCCTCGGAC-3′
|
|
