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. 2013 Mar 14;8(3):e59149. doi: 10.1371/journal.pone.0059149

Figure 3. GILZ deficiency leads to spermatogenic failure.

Figure 3

A: Testes were collected from 6, 10, 14, 20 and 30 day old and adult WT and Gilz KO mice, processed and stained with PAS. Scale bars represent 100 µM. B: The presence of mature sperm was detected in adult WT but not Gilz KO epididymides. Scale bars represent 100 µM. C: Representative images of TUNEL positive cells in 20 day old WT and Gilz KO testes. Scale bars represent 50 µM. D: Chromatin morphology of pachytene cells was examined using PAS staining (scale bars represent 50 µM) and SCP3 immunofluorescence in day 20 WT and Gilz KO testes.