Figure 6. Six Marker Immunohistochemistry of Connexins on Astrocytes Predominantly in the Parenchyma in the Retina of an Aged Rat.

Cx26 (blue, A), Cx30 (red, B), Cx43 (green, C), Cx45 (pale brown, D) were colocalised (E) on GFAP positive-astrocytes (pale purple, F). Connexin heterogeneity in gap junction plaques are indicated as follows: Cx26⁺/Cx43⁺ (large arrows), Cx26⁺/Cx45⁺ (small arrows), Cx26⁺/Cx30⁺/Cx43⁺ (curved arrows), Cx26⁺/Cx43⁺/Cx45⁺ (arrowheads). Scale bar, 50 µm (A–F).