Table 2. Information about the MYH9 non-coding SNPs and the non-synonymous APOL1 SNPs, genotyped in this study.

SNP	Risk haplotype	Forward primer	Reverse primer	Tm (°C)	Restriction enzyme	Cleavage products (bp)	Known associations in Caucasian populations
MYH9 rs4821481 (T/C)	E1	GAAGAGTACCCCGTATCTCAACAC	AGCATGAGGGCTTCTGCTTAACT	65	BfaI	132+115 (T allele)	1. Non-diabetic ESKD in Hispanic Americans [26] 2. Diabetic nephropathy in European Americans [33]
MYH9 rs4821480 (T/G)	E1	CTCATGCTTGTTCTTGAGCTTG	AGAACAGAAAGCAAGGAGAGCAG	66	DraI	261+291 (T allele)	1. Non-diabetic ESKD in Hispanic Americans [26] 2. Diabetic nephropathy in European Americans [33] 3. Non-diabetic CKD in European Americans [33]
MYH9 rs2413396 * (T/C)	S1	CCAGCACCTCCCCGTGA	GTGGAGAAGGTGATGCAGGAG	65	HaeII	109+21 (C allele)	1. Non-diabetic ESKD in Hispanic Americans [33]
MYH9 rs11089788 (C/A)	No strong LD (<0.3) with any neighboring SNPs, according to HapMap data	GATGTCCCATCCAATTGTTTTC	GATTATGGCCTAAAAAGGCAACTG	59	BslI	230+158 (C allele)	1. Association with serum creatinine levels in three Caucasian populations [33]
APOL1 E149K	-	CTGGTTTCTGAAAGAGTTTCCTCGGTTGAAAATT **	GCTGTGATTCCCAACTCCATCCCAGGTTCCAA	60	MseI	207+33 (K allele)	-
APOL1 M227I	-	CGTATTAGTCAGGATGGTCTCAATC	AAAGTTGGATATGTTCTCACCCAAA	65	BtgI	505+115 (M allele)	-
APOL1 R254K	-	CTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTTA **	ATTAACCCTCTCCACCTGTTCACCGCTTTCAGCTG	67	MseI	201+35+4 (K allele)	-

^*Genotyping of rs2413396 was performed according to [27].

^**Modified nucleotides for creating restriction sites are in bold and underlined.