Table 1.
Reactions associated with hybridization of nucleic acids strands
In bulk solution | Reaction |
---|---|
Non-specific hybridization | S + N ↔ S.N |
Specific target folding | S ↔ S’ |
At the microarray surface | |
Specific hybridization | P + S ↔ P.S |
Non-specific hybridization | P + N ↔ P.N |
Probe folding | P ↔ P’ |
During the washing phase | |
Dissociation of specific duplexes | P.S ↔ P (+ S) |
Dissociation of non-specific duplexes | P.N ↔ P (+ N) |