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. 2013 Jan 15;41(5):2869–2880. doi: 10.1093/nar/gks1464

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Nap1 tethering reduces the CENP-B assembly level on non-centromeric alphoid chromatin in vivo. (A) A schematic drawing of the alphoid^tetO ectopic integration site. (B) An alphoid^tetO ectopic integration cell line (61, HeLa-Int-03) was transfected with a set of tetR-EYFP-fusion expressing plasmids. The CENP-B signals on the ectopic site were apparently reduced by Nap1 tethering, but not by sNASP tethering. Arrowheads indicate alphoid^tetO DNA integration sites. The scale bars represent 10 µm.