Figure 7.

Thermostability and DNA binding dependence on C7 and C28. (A) The N-domain of Cbp2_Hb showing positions of C7 and C28 (sticks) and their separation distance. Mobility band shift assays with (B) Cbp2_Hb and (C) Cbp2_Hb^C7S/C28S mutant protein incubated with CRISPR-2r_Hb DNA at increasing temperatures. 40 nM Cbp2_Hb or Cbp2_Hb^C7S/C28S were incubated with 10 nM [³²P] 5′-end labelled CRISPR-2r_Hb DNA in 10 mM Tris-Cl, pH 7.6, 150 mM KCl, 10% glycerol for 20 min at 50°C to 95°C and electrophoresed in an 8% polyacrylamide gel. C indicates the CRISPR-2r_Hb DNA control. Incubation temperatures are indicated for each sample.