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. 2013 Jan 23;41(5):3257–3273. doi: 10.1093/nar/gkt007

Table 1.

LNAs and the corresponding sequences used in this work

Group 1 ONs Target sequence: 5′-AAGAAGAAAA-3′
    bisLNA-20 Cy3-tTcTtCtTtT-ctctc-tTtTcTtCtT
    bisLNA-20a Cy3-tTcTtCtTtT-ctctc-ttttcttctt
    bisLNA-20b Cy3-ttcttctttt-ctctc-tTtTcTtCtT
    bisLNA-20c Cy3-tTcTtCtTtT-s18-tTtTcTtCtT
    bisLNA-20d Cy3-tTcTtCtTtT-s18-tTtTcTtCtT (fully phosphorothioated)
    bisLNA-20e Cy3-tTcTtCtTtT-s18±s9-tTtTcTtCtT
    TFO-LNA-20 Cy3-tTcTtCtTtT
    WC-LNA-20 Cy5-tTtTcTtCtT
Group 2 ONs Target sequence: 5′-GCAGAGGGCGTGGGGGAAAAGAAAAAAGA-3′
    bisLNA-m30 Cy3-cCtTtTcTtTtTtCt-tctct-TcTtTtTtCtTtTcC
    bisLNA-m44 Cy3-CcTtTtCtTtTtTcT-tctct-tCtTtTtTcTtTtCcCccAcgCccTctGc
    bisLNA-m44a Cy3-CcTtTtCtTtTtTcT-tctct-TcTtTtTtCtTtTcCCccAcgCccTctGc
    bisLNA-m44b Cy3-cCtTtTcTtTtTtCt-tctct-TcTtTtTtCtTtTcCCccAcgCccTctGc
    bisLNA-m44c Cy3-CcTtTtCtTtTtTcT-tctct-TcTtTtTtCtTtTccCccAcgCccTctGc
    bisLNA-m44d Cy3-cCtTtTcTtTtTtCt-tctct-tCtTtTtTcTtTtCcCccAcgCccTctGc
    bisLNA-m44e Cy3-CcTtTtCtTtTtTcT-tctct-tCtTtTtTcTtTtCcCccACgCCcTCtGC
    bisLNA-m44-3t Cy3-tTtCtTtTtTcT-tctct-tCtTtTtTcTtTtCcCccAcgCccTctGc
    bisLNA-m44-7w Cy3-CcTtTtCtTtTtTcT-tctct-tCtTtTtTcTtTtCcCccAcgC
    WC-LNA-m30 Cy5-TcTtTtTtCtTtTcC
    WC-LNA-m44 Cy5-tCtTtTtTcTtTtCcCccAcgCccTctGc
    TFO-LNA-m30/44 Cy3-cCtTtTcTtTtTtCt
Group 3 ONs Target sequence: 5′-GGGAAAAAGAACGGAGGGAGGGA-3′
    bisLNA-m(P2)37 Cy3-CcTcCcTcCcT-tctctc-TcCcTcCcTcCgTtCtTtTtCcCgCc

LNA bases are in capital letters; DNA bases in small letters; linker sequence/region is underlined; s18 = hexaethylene glycyl linker; s18 + s9 = hexaethylene glycyl extended with a triethylene glycyl linker; target sequence of group 2 and 3 LNAs shown in italic corresponds to the complementary region of the extended Watson–Crick arm of bisLNA-m44.

Rational behind designations: m indicates a bisLNA targeting a MYC sequence, numbers correspond to number of nucleotides without those in the linker, t and w indicate the nature of the altered arm (TFO or WC, respectively) followed by the reduced number of nucleotides in the arm.