Figure 4.

Systematic bp substitution of the LysM consensus sequence to analyse sequence specificity of binding. In this figure, a representative example of such a binding analysis is shown for position −4, but analogous EMSAs have been performed and analysed for the other positions (Supplementary Table S2). (A) EMSAs of LysM binding to variants harbouring a single-bp substitution at position −4. The bp change is mentioned on top of each autoradiograph, as are LysM_Sa concentrations (in nM). DNA fragments were prepared by hybridizing complementary oligonucleotides. (B) Graphical representation of the systematic bp substitution experiment in an energy-normalized sequence logo. The height of the stack of letters corresponds to the information content (bits).