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HpDprA protects dsDNA from Type II restriction enzymes. 5′-end-labelled dsDNA (0.5 nM) either alone (lane 2) or pre-bound with increasing concentrations of HpDprA [5, 10, 15, 25, 50, 75, 150, 300, 500 and 1000 (nM), lanes 3–12] was incubated with 1 U of (A) R.HpyCH4V (B) R.HpyCH4III (C) R.Hpy188I for 60 min at 37°C. Lane 1: DNA alone. Figure 4A, lane 13: Δ indicates heat inactivated HpDprA (1000 nM).
