Figure 2.

Disruption of the XRCC1 BRCT1 domain results in the abolishment of XRCC1 recruitment to SSB but does not affect the recruitment of the protein to oxidative damage. (A) HeLa cells co-transfected with the WT XRCC1-DsRED monomer (red) and the mutant XRCC1(L360D)-GFP (green) were microirradiated in the presence or absence of Ro. Arrows indicate the site of irradiation. (B) Live-cell imaging of microirradiated HeLa cells expressing the XRCC1 mutant XRCC1(L360D)-GFP, in the absence of PARP inhibitors and in the presence or absence of Ro. Scale bar, 5 µm. Graph corresponds to mean values from 10 cells. Error bars represent the SEM.