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. 2013 Jan 25;41(5):3144–3161. doi: 10.1093/nar/gkt029

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Overexpression of inactive Ddk1 affects 7S DNA levels. Analysis of 7S DNA levels in parental 293 cells and their derivatives overexpressing wild-type or mutated (D251N K253A) Ddk1 for the indicated times. Each cell line was cultured in the presence of tetracycline. 7S DNA levels were examined using Southern blots. Graph presents the mean values obtained in two independent experiments. Error bars represent standard deviation. The signal arising from hybridization to the nuclear 28rDNA gene (nDNA) was used as a loading control. The P-values obtained in the Student’s t-test are indicated. ns, statistically insignificant; P > 0.05.