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. 2013 Jan 25;41(5):2950–2962. doi: 10.1093/nar/gkt032

Figure 4.

Figure 4.

The bipartite Gly-Arg rich domain of EBNA1 is required for chromatin decondensation. (a) Schematic representation of the EBNA1 protein: N terminus, amino acid 1–39; Gly-Arg repeat-1 (GR1), amino acid 39–89; Gly-Ala repeat (GA), amino acid 89–324; Gly-Arg repeat-2 (GR2), amino acid 324–379; Nuclear Localization Signal (NLS), amino acid 379–386; viral DBD, amino acid 459–598. (b) The GR1 and GR2 domains are necessary and sufficient for the chromatin decondensation effect. A03-1 cells were transfected with mCherry-LacR or mCherry-LacR fused to EBNA1 or the indicated EBNA1 subdomains. Decondensation of the array was observed on tethering of the full length EBNA1, EBNA1 deletion mutants containing both the GR1 and GR2 domains or the juxtaposed GR1 and GR2 domains alone. The relative size ±SE of the array in >30 cells analyzed in three independent experiments is shown, ***P < 0.001.