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. 2013 Jan 29;41(5):3010–3021. doi: 10.1093/nar/gkt035

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — LacI-MBD2 or LacI-MBD3 assemble MBD2–NuRD or MBD3–NuRD on the LacO array. (A) F42B8 (U2OS) cells were transfected with GFP-LacI-MBD2b or GFP-LacI-MBD3. GFP or Cherry signals were directly determined, and indirect immunofluorescence was carried out with antibodies against Mi2 (αMi-2), RbAp46 (αRbAp46) and HDAC1 (αHDAC1). Merged colour images show DAPI staining in addition. (B) Schematic representation and summary of the assembly of the different NuRD components. Green arrows indicate recruitment of the components pointed out by the arrowhead, whereas the grey arrows indicate no assembly.