Figure 5.

Genome-wide exosome chromatin association correlates strongly with active transcription. (A) Exosome associates preferentially with active promoters. Profile of S2 Rrp6 and Rrp40 input normalized ChIP-seq tag density around TSSs associated (red) or not associated (blue) with H3K4me3 and Pol II 250 bp upstream or 750 bp downstream of the TSS. (B) Matrices of Rrp6 and Rrp40 S2 ChIP signal across all annotated TSSs +/− 1 kb. Each row corresponds to an annotated gene, and red lines indicate grouping of rows into active, ambiguous or inactive TSSs. Rows are ordered by mean signal from highest to lowest within each subgroup. Active genes were defined as genes with Pol II and H3K4me3 within 250 bp upstream or 750 bp downstream of the TSS, ambiguous genes had one of these marks within this window, and inactive genes had neither mark in the same window. Signal intensity is shown in input-normalized enriched RPMMR. Color scale corresponding to enrichment is indicated (right).