Figure 2.
Fob-block cells require the MRX complex, but not homologous recombination for proper cell growth. Also, 10-fold serial dilutions of raffinose-grown cultures (non-induced) were spotted on glucose and galactose plates. (A) Shown are isogenic strains of wild-type (LBy-1), GAL-FOB1 (LBy-413) (B) Shown are isogenic strains of rad52Δ (LBy-108), GAL-FOB1 rad52Δ (LBy-588), rad51Δ (LBy-14), GAL-FOB1 rad51Δ (LBy-612) (C) Shown are isogenic strains of mre11Δ (LBy-605), GAL-FOB1 mre11Δ (LBy-756), xrs2Δ (LBy-80), GAL-FOB1 xrs2Δ (LBy-774), rad50Δ (LBy-615), GAL-FOB1 rad50Δ (LBy-581). (D) sir2Δ does not suppress the mre11Δ growth defect. Shown are isogenic strains of GAL-FOB1 (LBy-413), GAL-FOB1 mre11Δ (LBy-756), GAL-FOB1 sir2Δ (LBy-888), GAL-FOB1 mre11Δ sir2Δ (LBy-909).