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. 2013 Feb 1;41(5):3173–3189. doi: 10.1093/nar/gkt051

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Checkpoint activation is a direct cause of the ectopically placed RFB (A) Schematic representation of the modified Fob-block system with a TetO-array. (B) The ARS606–ARS607 region is fully replicated as measured by fluorescence microscopy on strain GAL-FOB1 eRFB TetR-TetO scc1-73 (LBy-868) and GAL-FOB1 eRFB TetR-TetO mre11Δ scc1-73 (LBy-880). Fluorescence (TetR-RFP) and differential interference contrast images (DIC) of representive cells. Arrowheads mark foci. (C) Quantification of cells containing two RFB foci and of cells with segregated foci. At each time point, 100–300 cells were examined for RFB foci.