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. 2013 Feb 1;41(5):3173–3189. doi: 10.1093/nar/gkt051

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Sir2 suppresses the checkpoint response in the rDNA. ISA was performed on asynchronous yeast cultures of GAL-FOB1 eRFB mre11Δ (LBy-649), GAL-FOB1 mre11Δ sir2Δ (LBy-909) and GAL-FOB1 sir2Δ (LBy-888) strains. Samples were taken before galactose induction and after 90 and 180 min of induction. The kinase assay was performed as described in ‘Material and Methods’ section and in Figure 6.