Figure 1.

Alternative splicing products of NCU01977. (A) 5′ region of precursor and alternatively spliced mRNAs for NCU01977. S1 through S4 identify alternative 5′ splice sites (GU dinucleotides) within intron 2. Gray, white and black bars represent exons, introns and TPP aptamers, respectively. AG dinucleotides represent 3′ splice sites. Arrows indicate the primer binding sites used for RT-PCR. (B) Agarose gel separation of RT-PCR products from NCU01977 mRNAs of N. crassa grown in the absence (−) or presence (+) of 30 μM thiamin. Bands I through V are DNA products from different splice variants. Asterisks denote RT-PCR products that were not sequenced. RT designates reverse transcription; M designates a lane containing DNA markers.