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. 2013 Mar 14;8(3):e57514. doi: 10.1371/journal.pone.0057514

Figure 4. DENV NS1 directly binds human C1q in an ELISA assay.

Figure 4

(A) Microtiter plates were coated with purified human C1q (10 µg/mL). After incubation with increasing concentrations of purified DENV NS1 that was purified from the supernatant of BHK cells, bound NS1 was detected using a specific conformational monoclonal anti-NS1 antibody. (B) Microtiter plates were coated with purified human C1q (10 µg/mL). After incubation with increasing concentrations of purified DENV NS1 that was purified from E. coli cells, bound NS1 was detected with a specific polyclonal anti-NS1 antibody. Error bars indicate standard deviation from three independent experiments, and asterisks indicate significant difference from the control mock or BSA. *p<0.05, **p<0.01, ***p<0.001.