Figure 7. Mitochondrial membrane depolarization and caspase-3 activity induced by Pho-s in Renca cells.

(a) Representative dot plots of cells stained with JC-1 dye are shown. The decrease of green fluorescence monomers correlates with the mitochondrial depolarization (Low), and cells exhibiting red fluorescence, which are viable cells (High). (b) The fluorescence intensities of JC-1 were determined by flow cytometry based on the dot plots analysis. (c) Caspase-3 activity increases were accompanied with a reduction of ΔΨ in Renca cells, induced by Pho-s and ST. The data are representative of three independent experiments performed in triplicate. Significant differences are indicated as: ***p<0.001 statistically different from the Pho-s versus untreated.