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. 2013 Mar 14;8(3):e58719. doi: 10.1371/journal.pone.0058719

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative pictures of the scratch wound assay. TGF-β1-treated MCF-7 cells migrated faster than controls. The black lines indicated size of the remaining wound. Data from three independent experiments were analyzed and presented as mean ± SD. (P<0.001). (B) Representative pictures in a transwell migration assay (top).The penetrated cells numbers of MCF-7 treated with TGF-β1 for 12 hrs were significantly increased as compared to controls without TGF-β1 (P<0.001). Representative pictures in a transwell invasion assay (bottom). TGF-β1 treatment led to more MCF-7 cells penetrating matrix than controls. (P = 0.002). The results in both transwell migration and invasion assays were obtained from three independent experiments.