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qRT-PCR evaluation of PsVPS1 gene (gray box) expression levels using hyphal RNA from recipient strain 6497, the control transformant (CK), and PsVPS1-silenced mutants (T22, T30, and T47). Expression was calibrated against the wild-type strain 6497 (value of 1). The qRT-PCR experiment was repeated three times with independent RNA isolations.
