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. 2013 Mar 14;8(3):e58623. doi: 10.1371/journal.pone.0058623

Figure 4. PsVPS1-silenced mutants showed reduced vegetative growth and vacuole morphology.

Figure 4

A. The transformants displayed retarded vegetative growth on 10% V8 medium. Pictures were taken 4 days after incubation. B. Colony diameter after 4 days of growth (p<0.01). C. Vps1 is involved in vacuole size. Transformants had smaller vacuoles than did wild-type strains. Wild-type and transformant cells were stained by CMAC. Hyphal vacuoles of wild-type and transformant strains after 2 days of growth in 10% V8 liquid medium were stained by CMAC. The dyed hyphae were examined using the Olympus ultraviolet excitation cube (330–385 nm excitation, >420 nm emission). Bar indicates 50 µm.