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. 2013 Mar 14;8(3):e58623. doi: 10.1371/journal.pone.0058623

Figure 5. PsVPS1 was required for normal zoospore germination.

Figure 5

A. Zoospores of the transformant displayed abnormal morphology. Zoospores from the wild-type and Psvps1-silenced mutants (T22, T30, and T47) were encysted by vortexing for 90 s, and cysts were incubated in clarified 5% V8 liquid medium at 25°C for 12 h, after which samples were photographed. B. Numbers of abnormal germinated cysts were counted at 12 h under the microscope. The ratio of the number of abnormal germinated cysts to the total number of cysts (germinated and ungerminated) was calculated. C. Silencing of PsVPS1 altered the distribution of β-1,4 polysaccharides in the cell wall. In silenced mutants, stained patches were detected along the hyphae and hyphal tips, indicative of abnormal polysaccharide accumulation. Bar indicates 20 µm.