FIGURE 3.

α-syn and Rab3a interaction occurs on presynaptic membranes. Lysates from synaptosomes expressing nontransgenic (Non Tg) (A), human wild type (WT) (B), A30P α-syn (C), or no α-syn (α-syn KO) (D) were separated into total, membrane-bound, and cytosolic proteins (Total, Memb, and Cyt) through hypotonic lysis. Membrane-associated and cytosolic fractions were immunoprecipitated (IP) either using anti-α-syn antibody (α-syn IP) or mouse pre-immune serum (Pre-immune). Western blots were probed for α-syn, Rab3a, Hsp90, and GDI. The blots shown are representative of three independent experiments. *, immunoglobulin heavy or light chain.