FIGURE 6.

Effects of GDI mutants on α-syn membrane binding. SH-SY5Y cells were infected with lentivirus expressing empty vector (Mock), His₆-tagged WT or mutant GDI (R240A and R218E) and stimulated for 10 min with a 55 mm high potassium buffer before fractionation into membrane-bound and cytosolic proteins. Upper panel shows representative Western blots of membrane (M) and cytosolic (Cyt) fractions probed for His₆-GDI and α-syn. The lower panel shows quantification of mean ± S.E. from four independent experiments of membrane-associated α-syn, respectively (*, p < 0.05, one-way ANOVA with Bonferroni post-test).