FIGURE 7.

Inhibition of HSP90 activity increases membrane-bound α-syn. SH-SY5Y neuroblastoma cells were treated with 50 μm radicicol (open circles) or 20 μm geldanamycin (closed squares) for 0–120 min prior to a 10-min stimulation with 55 mm potassium buffer. Cells were immediately separated into membrane and cytosolic fractions, which were probed for GDI, Rab3a, and α-syn. Quantification of membrane protein levels is shown as a percent of total expression (mean ± S.E.). *, significant change in membrane-bound protein as compared with the t = 0 drug treatment control (p < 0.005, one-way ANOVA with Bonferroni post-test, n = 5).