FIGURE 6.

Activation of ERK1/2 in fibroblasts and breast cancer cells is not abrogated by pitstop or dynasore. A and B, 10T1/2 cells were pretreated with vehicle, 10 μm pitstop, or 80 μm dynasore and then stimulated for the times indicated with 50 ng/ml FGF2 (A) or 10 ng/ml PDGF-BB (B). Cell lysates were analyzed by Western blotting for phosphorylated and total ERK1/2. C, MDA-MB-231 cells were pretreated with vehicle, 10 μm pitstop, or 80 μm dynasore and then stimulated for the times indicated with 100 ng/ml EGF. Cell lysates were analyzed by Western blotting for phosphorylated (Thr(P)-202/Tyr(P)-204) and total ERK1/2.