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. 2013 Jan 22;288(11):7519–7527. doi: 10.1074/jbc.M112.384453

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effects of BIN2 target site mutations on MKK4 activity in vitro and in planta. A, in vitro kinase assays using recombinant GST-BIN2 and GST-tagged wild-type or mutant variants of MKK4. AR, autoradiography; CBB, Coomassie Brilliant Blue; B, top, wild-type plants and T1 plants expressing 35S:MKK4^T234A-YFP. Bottom, Western blot analysis of the studied lines with anti-YFP antibody. C, SEM image of the abaxial cotyledon epidermis of a representative plant, which strongly expresses 35S:MKK4^T234A-YFP, 12 days after germination. The scale bar represents 30 μm.