FIGURE 6.

BIN2 acts upstream of MKK4 in stomata patterning. A, top, wild-type and homozygous lines expressing 35S:MKK4-YFP. Bottom, Western blot analysis of the studied lines with anti-YFP antibody. B, analysis of MKK4-YFP subcellular localization in A. thaliana plants stably expressing 35S:MKK4-YFP. C, phenotypes of seedlings with or without 1 μm Brz. D, hypocotyl length of seedlings used for quantification of stomata clustering. Error bars indicate S.D. E, the frequency of stomata clustering in wild-type and 35S:MKK4-YFP plants in the presence or absence of 1 μm Brz as determined by light microscopy. The results are shown in percentages. p values derived of a χ²-test are given.