FIGURE 1.

The structures of l-canavanine and l-arginine and the tertiary structure and multiple alignment of MazF-bs. A and B, structure of l-canavanine (A) and l-arginine (B). C, alignments of MazF-bs with other MazF homologues from Staphylococcus aureus (MazF-sa) and E. coli (MazF-ec), PemK from E. coli (PemK-ec), ChpBK from E. coli (ChpBK-ec), MazF-mt1 to -mt7 from Mycobacterium tuberculosis, MazF-mx from Myxococcus xanthus, and MazF-hw from Haloquadra walsbyi. White and black boxes indicate β-sheets and α-helices on the basis of the secondary structure of MazF-bs, respectively. ClustalW2 was used for multiple sequence alignments. All Arg residues in MazF-bs are indicated by black shading and homologous residues to Arg by gray shading. D, crystal structure of MazF-bs dimer (Protein Data Bank ID code: 1NE8) imaged in PyMOL. Green and blue colors show as an individual monomer, and Arg residues are colored in magenta.