FIGURE 3.

Both negatively and positively charged residues in the periplasmic region can function as YidC determinants. E. coli JS7131 cells bearing different plasmids were grown under YidC expression or YidC depletion conditions, labeled, and analyzed for translocation of the N-tail using the protease accessibility assay as described in the legend to Fig. 2. The plasmids encoded the proteins Pf3-LepTM V4E, V15D, V4Q, V15N, V4R, V15R, V15K, V4E/V15D, and V4R/V15R. A representative OmpA immunoprecipitation data under YidC+ or YidC− conditions is shown at the bottom. PK, proteinase K.