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. 2013 Jan 28;288(11):7738–7755. doi: 10.1074/jbc.M112.428078

FIGURE 6.

FIGURE 6.

Trophic effects of MEF feeder cells reconstituted GRK2-dependent growth control in vitro. A, cell proliferation analysis of HEK cells plated on mitomycin-inactivated MEF feeder cells showed decreased cell proliferation of GRK2-expressing HEK cells compared with HEK control cells and enhanced cell proliferation of dominant-negative GRK2-K220R-expressing cells. As indicated, EGF-supplemented cells were treated without or with the MEK inhibitor PD0325901. Cell proliferation is expressed as percentage of control, i.e. MEK inhibitor-treated control cells (set to 100%). Bars represent mean ± S.D., n = 3 (a, p = 0.0022; and b, p = 0.0018 versus Cont. without MEK inhibitor; c, p = 0.1515; and d, p = 0.1029 versus Cont. with MEK inhibitor). B, nuclear phospho-ERK1/2 levels of HEK cells plated on mitomycin-inactivated MEF feeder cells. The left and middle panels show a representative experiment and the right panel presents the quantitative evaluation of three different experiments without MEK inhibitor (± S.D., n = 3; a, p = 0.0113, and b, p = 0.0312 versus Cont.).