Inserts and primers
A, schematic diagram of NMHC mRNA. Locations of C1 and C2 inserts and primers are denoted with triangles and arrows, respectively. Dashed lines represent nucleotide number. Note that C1 exon (24 nucleotides in size) and C2 exon (123 nucleotides in size) are included after nucleotides 681 and 1908 of NMHC II-C mRNA, respectively. B, summary of RT-PCR analysis from Figs. 1 and 2 for each NMHC II-C isoform in Neuro-2a cells using isoform-specfic primers. ND, not detectable.