TABLE 2.

Percentage of GFP-positive cells having neurites and their average neurite lengths

Plasmid DNA containing GFP-tagged NMHC II-C isoform was transfected into 24-h post-siRNA-treated cells. After 72 h of post-DNA transfection, GFP-positive cells were used for quantification. Data are represented as means ± S.E. from three independent experiments. n > 100 cells from each experiment.

	GFP alone	NMHC II-C0-GFP	NMHC II-C1-GFP	NMHC II-C2-GFP	NMHC II-C1C2-GFP
Round cells (%)	90 ± 2.1	85.2 ± 2	86.1 ± 2	44.4 ± 1.9	35.7 ± 1.95
Cells with <2 neurites (%)	3.3 ± 1	7.4 ± 1.6	8.4 ± 1	29.6 ± 3	38.1 ± 2.15
Cells with >2 neurites (%)	6.7 ± 1	7.4 ± 1.1	5.5 ± 0.9	26 ± 1.95	26.2 ± 2.1
Average neurite length (μm)	7.35 ± 1.2	6.32 ± 0.9	6.85 ± 1.5	35.57 ± 3.2	48.43 ± 4.2