Skip to main content
. 2013 Jan 25;288(11):7907–7917. doi: 10.1074/jbc.M112.441048

FIGURE 1.

FIGURE 1.

TPPP1 inhibits cell proliferation. TPPP1 overexpression reduces cell proliferation. A, the proliferation of U2OS cells stably expressing FLAG-TPPP1 (TPPP1) or vector were analyzed. Assays were performed at daily intervals for a 5-day period as described under “Experimental Procedures” (p < 0.0001). Immunoblot analysis of U2OS cells stably expressing F-TPPP1 or vector that were probed for FLAG, TPPP1, and GAPDH (loading control) showed a 7-fold increase in TPPP1 expression compared with vector. B, TPPP1 knockdown increases cell proliferation. Proliferation assays were performed for 5 days as described under “Experimental Procedures” (p < 0.0001). Immunoblot analysis of TPPP1 knockdown probed for TPPP1 and GAPDH (loading control). Data in A and B were analyzed by two-way analysis of variance with Bonferroni's post-test.