TABLE 1.
Construct | Mutant sites | Mutant type |
---|---|---|
Tppp13Ala/4Ala | S32A/S107A/S159A-T14A/S18A/S45A/S160A | Dual phosphoinhibitory |
Tppp13Ala/4Glu | S32A/S107A/S159A-T14E/S18E/S45E/S160E | Rock phosphoinhibitory/Cdk phosphomimetic |
Tppp13Glu/4Ala | S32E/S107E/S159E-T14A/S18A/S45A/S160A | Rock phosphomimetic/Cdk phosphoinhibitory |
Tppp13Glu/4Glu | S32E/S107E/S159E-T14E/S18E/S45E/S160E | Dual phosphomimetic |