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. 2013 Jan 25;288(11):7907–7917. doi: 10.1074/jbc.M112.441048

TABLE 1.

Dual ROCK and Cdk phosphoinhibitory and phosphomimetic TPPP1 mutants

Construct Mutant sites Mutant type
Tppp13Ala/4Ala S32A/S107A/S159A-T14A/S18A/S45A/S160A Dual phosphoinhibitory
Tppp13Ala/4Glu S32A/S107A/S159A-T14E/S18E/S45E/S160E Rock phosphoinhibitory/Cdk phosphomimetic
Tppp13Glu/4Ala S32E/S107E/S159E-T14A/S18A/S45A/S160A Rock phosphomimetic/Cdk phosphoinhibitory
Tppp13Glu/4Glu S32E/S107E/S159E-T14E/S18E/S45E/S160E Dual phosphomimetic