TABLE 2.
Primers used for construction of the mrx1 (NCgl0808) and nrdh (NCgl2445) disruption and deletion mutants
| Primer name | Sequence (restriction sites are underlined) | Purpose |
|---|---|---|
| mrx1int-Fw | TAATTTAAA GGATCCACCTCATCGACGTCGACC (DraI/BamHI) | mrx1 disruption |
| mrx1int-Rv | TAATTTAAA AAGCTTATCGGAGTAGCGCAC (DraI/HindIII) | mrx1 disruption |
| mrx1sac-upFw | TAATTTAAA GAATTCCATTGATGCAGTTCAATGGGGGTAAATTG (DraI/EcoRI) | mrx1 deletion |
| mrx1sac-upRv | GTTTAAGGAGCAAACTCTAGAGCAAAAAAGAGCCTTTC (XbaI) | mrx1 deletion |
| mrx1sac-downFw | GAAAGGCTCTTTTTTGCTCTAGAGTTTGCTCCTTAAAC (XbaI) | mrx1 deletion |
| mrx1sac-downRv | TAATTTAAA AAGCTT ATTTATGACAACCACAAGGAACAGGTCG (DraI/HindIII) | mrx1 deletion |
| mrx2int-Fw | TAATTTAAA GGATCCTGCGTCCAGTGCAATGCCA (DraI/BamHI) | nrdh disruption |
| mrx2int-Rv | TAATTTAAA AAGCTTGGGAGCCATCTGCAACGACAA (DraI/HindIII) | nrdh disruption |
| mrx2sac-upFw | TAA TTTAAA GGATCC ATGCGAAAGTGAGTATTGCG (DraI/BamHI) | nrdh deletion |
| mrx2sac-upRv | CGATTAAACAGGTCGTGCCTCTAGAGACCTTCCCTTATCGGC (XbaI) | nrdh deletion |
| mrx2sac-downFw | GCCGATAAGGGAAGGTCTCTAGAGGCACGACCTGTTTAATCG (XbaI) | nrdh deletion |
| mrx2sac-downRv | TAATTTAAAAAGCTTAAACCTGATGACTTGTGG (DraI/HindIII) | nrdh deletion |