Figure 2. Expression of surface markers associated with cell signaling and cytokine production in LPS-stimulated, purified B cells from SMAD−/− mice.

Aliquots of splenic B cells, purified using negative selection and identified above as B220⁺, were cultured for 24 h in media containing 1 μg/mL LPS. (A) Cell surface marker expression of LPS-stimulated B cells in SMAD−/− mice fed the CON (open bars) or DFO (solid bars) diet. (B) Cytokine production in the supernatants of LPS-stimulated B cells from SMAD−/− mice fed the CON or DFO diet was assayed. Data are represented as mean ± sem; n = 8–10. Data indicate significant differences between the DFO diet and the CON diet: *P < 0.05; **P < 0.01.