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Only misfolded HEL is presented on I-Ak. Plasmids containing wild-type and mutant HEL were transfected into 293T cells with I-Ak and GFP. Plasmids containing membrane-tethered wild-type HEL (Membrane wtHEL) and cysteine-mutant HEL (mutHEL) were transfected with GFP plasmids. Cell surface HEL on GFP-positive cells was analyzed using HyHEL10 and Gloop4 mAbs. All data are representative of three independent experiments.
