Figure 3. Cell proliferation and adhesion analysis on the six silicon substrates.

(A) The proliferation rate of mouse 3T3 fibroblasts is measured on the six substrates at 1, 3 and 7 days post seeding, using EdU labeling. The proliferation rate was measured as the percentage of area occupied by the cells to the total surface area. (B) The surface area covered by the cell at day 1 post seeding measured on the six substrates. (C) The number of paxillin clusters measured via widefield fluorescent microscopy.