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. 2013 Feb 4;5(3):397–412. doi: 10.1002/emmm.201202046

Figure 2. TFEB gene transfer in PiZ mice promoted hepatic ATZ clearance.

Figure 2

  1. PAS staining was markedly reduced in livers from PiZ mice at 1 month (1 mo) and 6 months (6 mo) after the injection of HDAd-TFEB compared to saline or HDAd-AFP injected mice (magnifications 20× and 40×).
  2. ATZ immunofluorescence of livers from HDAd-TFEB injected mice showed a marked reduction of ATZ globules compared to controls at 1 and 6 months post-injection (magnification 20×). Representative images from experimental groups including at least n = 5 mice per group are shown.
  3. ELISA for ATZ on hepatic extracts showed a statistically significant reduction of ATZ in HDAd-TFEB injected mice at 1 and 6 months post-injection compared to HDAd-AFP and saline injected controls (at least n = 5 per group).
  4. TFEB real time PCR on liver mRNA showed TFEB expression as fold increase over saline at 1 and 6 months post-injection in HDAd-TFEB injected mice (at least n = 5 per group). Averages ± standard deviations are shown. *p < 0.05.