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. 2013 Feb 4;5(3):397–412. doi: 10.1002/emmm.201202046

Figure 7. Increased ATZ in autophagolysosomes of HDAd-TFEB injected PiZ mice.

Figure 7

Livers from either HDAd-AFP injected (A,B,D,E) or HDAd-TFEB injected (C,F,G) mice were prepared for immuno-EM of ATZ as described in Materials and Methods Section. Scale bars: 1.8 µm (A), 520 nm (B), 320 nm (C), 125 nm (F), 110 nm (D,E,G).

  • A. Region of the hepatocyte cytoplasm exhibits large membrane bound inclusion (asterisk) similar to that shown in Fig 6B.
  • B. Higher magnification from the region outlined by dash box in A reveals dense gold ATZ labelling over inclusion body (asterisk).
  • C. Arrows indicate diffuse ATZ signal along the RER membranes of hepatocyte in HDAd-TFEB-treated mice.
  • D,E. Lysosomes (arrows) and their intraluminal vesicles (arrowheads) show little or no ATZ in control saline-injected animals.
  • F,G. Liver cells from HDAd-TFEB injected mice exhibit ATZ-corresponding gold particles within the lysosome-like structures (arrows) where ATZ signal is frequently associated with intraluminal vesicles (arrowheads).