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. 2013 Mar-Apr;33(2):132–142. doi: 10.3747/pdi.2011.00234

Figure 5.

Figure 5

— Immunohistochemistry for F4/80 and monocyte chemoattractant protein-1 (MCP-1) and southwestern histochemistry for activated nuclear factor κB (NF-κB). (A) In the chlorhexidine gluconate (CG) group, a large number of F4/80-positive macrophages were present in the submesothelial zone. (B) Fewer macrophages were observed in the CG plus 22-oxacalcitriol (OCT) group. (C) Cells expressing MCP-1 were present in thickened peritoneal tissues in the CG group. (D) Compared with the CG group, the CG+OCT group showed a reduced number of MCP-1-positive cells. (E,F) The panels of southwestern histochemistry for activated NF-κB were evaluated using an image analyzer. The red color was assigned to positive cells. (E) In the CG group, cells positive for activated NF-κB were detected in the submesothelial compact zone. (F) The number of cells positive for activated NF-κB was significantly lower in the OCT group. (A-F) Original magnification 200×.