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. 2013 Feb 9;13:10. doi: 10.1186/1472-6750-13-10

Table 2.

Phenolic compound contents in the cell wall fractions

μg/g DW
Fraction   Vanillic acid Vanillin Coumaric acid Syringic aldehyde Ferulic acid Vitexin
WSF
Nike
0.360 ± 0.048
0.108 ± 0.028
0.169 ± 0.065
0.152 ± 0.052
0.744 ± 0.142
0.757 ± 0.014
B14
0.260 ± 0.007
0.150 ± 0.045
0.103 ± 0.036
0.09 ± 0.013
0.811 ± 0.336
0.930 ± 0.322
CSF
Nike
0.071 ± 0.003
0.009 ± 0.001
0.038 ± 0.004
0.016 ± 0.003
0.162 ± 0.006
0.213 ± 0.014
B14
0.104 ± 0.032
0.016 ± 0.003*
0.055 ± 0.011
0.032 ± 0.018
0.295 ± 0.055*
0.326 ± 0.053*
NSF
Nike
0.258 ± 0.021
0.034 ± 0.009
0.150 ± 0.021
0.055 ± 0.009
0.635 ± 0.021
1.085 ± 0.085
B14
0.201 ± 0.014*
0.043 ± 0.008
0.12 ± 0.014
0.042 ± 0.003*
0.622 ± 0.012
1.022 ± 0.074
K1SF
Nike
1.771 ± 0.115
1.196 ± 0.240
0.431 ± 0.041
1.212 ± 0.071
2.306 ± 0.176
3.349 ± 0.270
B14
1.886 ± 0.530
1.917 ± 0.505
0.593 ± 0.032**
0.895 ± 0.11*
2.925 ± 0.281*
4.044 ± 0.361*
K4SF Nike
0.477 ± 0.286
0.079 ± 0.01
0.046 ± 0.012
0.116 ± 0.048
0.145 ± 0.050
0.185 ± 0.047
B14 1.597 ± 0.199* 0.336 ± 0.034** 0.127 ± 0.009** 0.370 ± 0.045** 0.563 ± 0.073** 0.256 ± 0.034

UPLC determination of the vanillic acid, coumaric acid, ferulic acid, vanillin, syringic aldehyde and vitexin contents in the cell wall fractions (WSF – water-soluble fraction, CSF – CDTA-soluble fraction, NSF – Na2CO3-soluble fraction, K1SF – 1 M KOH-soluble fraction and K4SF – 4 M KOH-soluble fraction) for fibres from transgenic flax line B14 and non-transgenic control flax Nike. Analyses were performed as described in the Methods section. Data represents the mean values ± SD from three independent experiments. The significance of the differences between the means was determined using Student’s t test (* - P < 0.05, ** - P < 0.01).