Table 2.
μg/g DW | |||||||
---|---|---|---|---|---|---|---|
Fraction | Vanillic acid | Vanillin | Coumaric acid | Syringic aldehyde | Ferulic acid | Vitexin | |
WSF |
Nike |
0.360 ± 0.048 |
0.108 ± 0.028 |
0.169 ± 0.065 |
0.152 ± 0.052 |
0.744 ± 0.142 |
0.757 ± 0.014 |
B14 |
0.260 ± 0.007 |
0.150 ± 0.045 |
0.103 ± 0.036 |
0.09 ± 0.013 |
0.811 ± 0.336 |
0.930 ± 0.322 |
|
CSF |
Nike |
0.071 ± 0.003 |
0.009 ± 0.001 |
0.038 ± 0.004 |
0.016 ± 0.003 |
0.162 ± 0.006 |
0.213 ± 0.014 |
B14 |
0.104 ± 0.032 |
0.016 ± 0.003* |
0.055 ± 0.011 |
0.032 ± 0.018 |
0.295 ± 0.055* |
0.326 ± 0.053* |
|
NSF |
Nike |
0.258 ± 0.021 |
0.034 ± 0.009 |
0.150 ± 0.021 |
0.055 ± 0.009 |
0.635 ± 0.021 |
1.085 ± 0.085 |
B14 |
0.201 ± 0.014* |
0.043 ± 0.008 |
0.12 ± 0.014 |
0.042 ± 0.003* |
0.622 ± 0.012 |
1.022 ± 0.074 |
|
K1SF |
Nike |
1.771 ± 0.115 |
1.196 ± 0.240 |
0.431 ± 0.041 |
1.212 ± 0.071 |
2.306 ± 0.176 |
3.349 ± 0.270 |
B14 |
1.886 ± 0.530 |
1.917 ± 0.505 |
0.593 ± 0.032** |
0.895 ± 0.11* |
2.925 ± 0.281* |
4.044 ± 0.361* |
|
K4SF | Nike |
0.477 ± 0.286 |
0.079 ± 0.01 |
0.046 ± 0.012 |
0.116 ± 0.048 |
0.145 ± 0.050 |
0.185 ± 0.047 |
B14 | 1.597 ± 0.199* | 0.336 ± 0.034** | 0.127 ± 0.009** | 0.370 ± 0.045** | 0.563 ± 0.073** | 0.256 ± 0.034 |
UPLC determination of the vanillic acid, coumaric acid, ferulic acid, vanillin, syringic aldehyde and vitexin contents in the cell wall fractions (WSF – water-soluble fraction, CSF – CDTA-soluble fraction, NSF – Na2CO3-soluble fraction, K1SF – 1 M KOH-soluble fraction and K4SF – 4 M KOH-soluble fraction) for fibres from transgenic flax line B14 and non-transgenic control flax Nike. Analyses were performed as described in the Methods section. Data represents the mean values ± SD from three independent experiments. The significance of the differences between the means was determined using Student’s t test (* - P < 0.05, ** - P < 0.01).