Table 3.
Effect of NVP-AEW541 (IC70) on the cell cycle distribution of pancreatic cancer cell lines
| Cell line/treatment | Sub-G1 | G0/G1 | S | G2/M |
|---|---|---|---|---|
|
BxPc3 |
|
|
|
|
| Control |
7.7±1.4 |
55.6±2.3 |
25.4±5 |
10.8±1.8 |
| NVP-AEW541 |
7.9±2.7 |
69.7±4.5* |
15.7±2.9 |
6.2±1.1 |
|
AsPC-1 |
|
|
|
|
| Control |
1±0.02 |
46.1±0.3 |
31.8±0.2 |
19.5±0.1 |
| NVP-AEW541 |
3.9±0.2* |
47.1±0.4 |
35.7±1.5 |
14±2.6 |
|
Capan-1 |
|
|
|
|
| Control |
3.8±0.3 |
50.6±2.5 |
25.5±3.1 |
18.6±1.1 |
| NVP-AEW541 |
7±0.2* |
53.6±1.3 |
23±1.8 |
15.1±1.4 |
|
PT45 |
|
|
|
|
| Control |
2.3±0.6 |
74.4±4.3 |
12.3±2.8 |
10.1±1 |
| NVP-AEW541 |
10.2±2.7* |
59.8±1.4* |
19.7±4.8 |
9.9±2.4 |
|
Miapaca-2 |
|
|
|
|
| Control |
4.6±2.6 |
76.1±2.2 |
10±1.3 |
8.6±0.5 |
| NVP-AEW541 |
5.5±2.7 |
81±1.3 |
9.4±0.8 |
4.5±3.7 |
|
PANC1 |
|
|
|
|
| Control |
5.6±1.5 |
48.3±4.6 |
11.6±0.1 |
32.7±4.7 |
| NVP-AEW541 |
9.3±0.5 |
72.4±2.1* |
12.1±0.3 |
4.9±0.4* |
|
FA6 |
|
|
|
|
| Control |
13.8±2 |
48.3±3.1 |
25.4±4.8 |
11.2±0.4 |
| NVP-AEW541 | 36.8±2.8* | 33.2±1.2* | 22.2±1.3 | 6.9±2.2 |
Each population is expressed as percentage of gated cells (mean of three independent experiments ± S.D).* depicts statistically significant difference (p<0.05) compared to control values.