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TSA can reverse cytokine profiles in T cell differentiation in vitro. BAL fluid and serum were harvested 24 hours after the last challenge. The concentration of IL-4 (A) and IFN-γ (B) in the BAL fluid and serum in OVA-immunized rats or non-immunized rats were measured directly by ELISA. Lung T cells isolated from OVA-immunized rats were cultured and IL-4 (A) and IFN-γ (B) in the supernatant were measured 24 h after incubation with or without TSA (2.5 ng/ml).
